Coding

Part:BBa_K2235004:Design

Designed by: Achilleas Fardellas   Group: iGEM17_Stockholm   (2017-09-27)


Colicin E2 immunity protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been optimized for E.coli strain, K12 using the codon optimization tool in IDT website.


Source

Obtained as a part of a G-block and it was PCR amplified and cloned into the plasmid pSB1C3.

References

1. Kleanthous C, Walker D. Immunity proteins: enzyme inhibitors that avoid the active site. Trends in Biochemical Sciences. 2001;26(10):624-631.